Rat Claudin 2 (CLDN2) ELISA Kit | CLDN2 elisa kit

Rat Claudin 2 (CLDN2) ELISA Kit

Cat. Num. AAA2707680

• Reactivity: Rat
• Synonyms: Claudin 2 (CLDN2); Rat Claudin 2 (CLDN2) ELISA Kit; SP82; CLDN2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Claudin 2 (CLDN2).; No significant cross-reactivity or interference between Claudin 2 (CLDN2) and analogues was observed.
• Sequence Length: 230

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.113ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 2 (CLDN2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 2 (CLDN2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLDN2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 2 (CLDN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 2 (CLDN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 2 (CLDN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 2 (CLDN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15680159
• NCBI GeneID: 9075
• NCBI Official Full Name: Claudin 2
• NCBI Official Synonym Full Names: claudin 2
• NCBI Official Symbol: CLDN2
• NCBI Protein Information: claudin-2
• Protein Family: Claudin

NCBI Description

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]