Typical Testing Data/Standard Curve (for reference only)
Rat Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) ELISA Kit | CYP11A1 elisa kit
Rat Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CYOX4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CYOX4 antibody and an CYOX4-HRP conjugate. The assay sample and buffer are incubated together with CYOX4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYOX4 concentration since CYOX4 from samples and CYOX4-HRP conjugate compete for the anti-CYOX4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYOX4 from the sample, fewer sites are left to bind CYOX4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYOX4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.
Protein type: EC 1.14.15.6; Mitochondrial; Oxidoreductase; Lipid Metabolism - C21-steroid hormone
Chromosomal Location of Human Ortholog: 15q23-q24
Cellular Component: mitochondrial crista; mitochondrion; mitochondrial matrix; perikaryon
Molecular Function: cholesterol monooxygenase (side-chain-cleaving) activity; iron ion binding; cholesterol binding; heme binding
Biological Process: steroid metabolic process; response to alkaloid; maternal process involved in pregnancy; dibenzo-p-dioxin metabolic process; response to insecticide; response to L-ascorbic acid; estrogen biosynthetic process; response to salt stress; response to vitamin E; Leydig cell differentiation; biphenyl metabolic process; cerebellum development; response to gamma radiation; response to corticosterone stimulus; response to drug; cholesterol metabolic process; progesterone biosynthetic process; fractalkine metabolic process; hippocampus development; phthalate metabolic process; mating behavior; vitamin D metabolic process; Schwann cell differentiation; response to genistein; response to hydrogen peroxide; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; sterol metabolic process; granulosa cell differentiation; phenol metabolic process
Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete
Research Articles on CYP11A1
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Product Notes
The Rat CYP11A1 cyp11a1 (Catalog #AAA7249834) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7249834 ELISA Kit recognizes Rat CYP11A1. It is sometimes possible for the material contained within the vial of "Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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