Rat CENPB ELISA Kit | CENPB elisa kit
Rat CENPB (Centromere Protein B) ELISA Kit
Reactivity
Rat
Synonyms
CENPB; Rat CENPB (Centromere Protein B) ELISA Kit; CENPB elisa kit
Reactivity
Rat
Specificity
This kit recognizes natural and recombinant Rat CENPB. No significant cross-reactivity or interference between Rat CENPB and analogues was observed.
Sequence Length
622
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CENPB elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CENPB concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CENPB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CENPB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CENPB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CENPB. You can calculate the concentration of CENPB in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CENPB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CENPB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CENPB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CENPB. You can calculate the concentration of CENPB in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
65,381 Da
NCBI Official Full Name
Cenpb protein
NCBI Official Synonym Full Names
centromere protein B
NCBI Official Symbol
Cenpb
NCBI Protein Information
major centromere autoantigen B; CENP-B
UniProt Protein Name
Major centromere autoantigen B
Protein Family
UniProt Gene Name
Cenpb
UniProt Synonym Gene Names
Cenp-b; CENP-B
UniProt Entry Name
CENPB_MOUSE
Uniprot Description
CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
Protein type: DNA-binding
Cellular Component: chromosome; nucleus; chromosome, pericentric region
Molecular Function: nucleic acid binding; DNA binding; chromatin binding
Biological Process: regulation of transcription, DNA-dependent
