Rat Cathepsin B (CTSB) ELISA Kit | CTSB elisa kit

Rat Cathepsin B (CTSB) ELISA Kit

Cat. Num. AAA2704830

• Gene Names: CTSB; CATB
• Reactivity: Rat
• Synonyms: Cathepsin B (CTSB); Rat Cathepsin B (CTSB) ELISA Kit; CTS-B; APPS; CPSB; APP secretase; CTSB elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin B (CTSB).; No significant cross-reactivity or interference between Cathepsin B (CTSB) and analogues was observed.
• Sequence Length: 335

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.062ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin B (CTSB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin B (CTSB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin B (CTSB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin B (CTSB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin B (CTSB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin B (CTSB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27806671
• NCBI GeneID: 281105
• NCBI Accession #: NP_776456.1
• NCBI GenBank Nucleotide #: NM_174031.2
• UniProt Accession #: P07688; Q3ZC03
• Molecular Weight: 36,661 Da
• NCBI Official Full Name: cathepsin B
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: CTSB
• NCBI Official Synonym Symbols: CATB
• NCBI Protein Information: cathepsin B
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: CTSB

Uniprot Description

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.