Rat Caspase 8 (CASP8) ELISA Kit | CASP8 elisa kit
Rat Caspase 8 (CASP8) ELISA Kit
Gene Names
Casp8; CASP-8
Reactivity
Rat
Synonyms
Caspase 8 (CASP8); Rat Caspase 8 (CASP8) ELISA Kit; CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog; CASP8 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of CASP8. No significant cross-reactivity or interference between CASP8 and analogues was observed.
Sequence Length
482
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.053ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CASP8 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CASP8 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Standard Curve/Testing Data
Related Product Information for CASP8 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP8 in rat Tissue homogenates, cell lysates and other biological fluids
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CASP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CASP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CASP8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CASP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CASP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CASP8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,339 Da
NCBI Official Full Name
caspase-8
NCBI Official Synonym Full Names
caspase 8
NCBI Official Symbol
Casp8
NCBI Official Synonym Symbols
CASP-8
NCBI Protein Information
caspase-8
UniProt Protein Name
Caspase-8
Protein Family
UniProt Gene Name
Casp8
UniProt Synonym Gene Names
CASP-8
NCBI Description
member of the cysteine-aspartic acid protease (caspase) familythat mediates the terminal stage of apoptosis; involved in apoptosis induced by Fas and other stimuli [RGD, Feb 2006]
Uniprot Description
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
