Dog Calcitonin (CT) ELISA Kit | CT elisa kit

Dog Calcitonin (CT) ELISA Kit

Cat. Num. AAA2700159

• Gene Names: CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• Reactivity: Dog
• Synonyms: Calcitonin (CT); Dog Calcitonin (CT) ELISA Kit; CALCA; CALC1; CGRP-I; CGRP1; KC; CCP; PDN-21; Katacalcin; Calcitonin-Related Polypeptide Alpha; Calcitonin carboxyl-terminal peptide; CT elisa kit

• Reactivity: Dog
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calcitonin (CT).; No significant cross-reactivity or interference between Calcitonin (CT) and analogues was observed.
• Sequence Length: 141

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 12.35-1000pg/mL
• Sensitivity: < 5.28pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin (CT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin (CT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CT elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Calcitonin (CT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Calcitonin (CT) and unlabeled Calcitonin (CT) (Standards or samples) with the pre-coated antibody specific to Calcitonin (CT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin (CT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Calcitonin (CT) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4502545
• NCBI GeneID: 796
• NCBI Accession #: NP_001732.1
• NCBI GenBank Nucleotide #: NM_001741.2
• UniProt Accession #: P01258; Q13935; Q13937; Q52LX7; B7ZL39; P06881
• Molecular Weight: 13,898 Da
• NCBI Official Full Name: calcitonin isoform CT preproprotein
• NCBI Official Synonym Full Names: calcitonin related polypeptide alpha
• NCBI Official Symbol: CALCA
• NCBI Official Synonym Symbols: CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• NCBI Protein Information: calcitonin; calcitonin gene-related peptide 1
• UniProt Protein Name: Calcitonin
• UniProt Gene Name: CALCA
• UniProt Synonym Gene Names: CALC1; CCP

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.