Rat C-Type Natriuretic Peptide (CNP) ELISA Kit | CNP elisa kit

Rat C-Type Natriuretic Peptide (CNP) ELISA Kit

Cat. Num. AAA2702350

• Gene Names: NPPC; CNP; CNP2
• Reactivity: Rat
• Synonyms: C-Type Natriuretic Peptide (CNP); Rat C-Type Natriuretic Peptide (CNP) ELISA Kit; CNP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of C-Type Natriuretic Peptide (CNP).; No significant cross-reactivity or interference between C-Type Natriuretic Peptide (CNP) and analogues was observed.
• Sequence Length: 126

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 11.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Natriuretic Peptide (CNP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Natriuretic Peptide (CNP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Natriuretic Peptide (CNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Natriuretic Peptide (CNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Natriuretic Peptide (CNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Natriuretic Peptide (CNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13249346
• NCBI GeneID: 4880
• NCBI Accession #: NP_077720.1
• NCBI GenBank Nucleotide #: NM_024409.3
• UniProt Accession #: P23582; Q4ZG41
• Molecular Weight: 13,246 Da
• NCBI Official Full Name: C-type natriuretic peptide preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide C
• NCBI Official Symbol: NPPC
• NCBI Official Synonym Symbols: CNP; CNP2
• NCBI Protein Information: C-type natriuretic peptide
• UniProt Protein Name: C-type natriuretic peptide
• Protein Family: 2',3'-cyclic-nucleotide 3'-phosphodiesterase
• UniProt Gene Name: NPPC
• UniProt Synonym Gene Names: CNP2

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cardiac natriuretic peptides CNP-53, CNP-29 and CNP-22, which belong to the natriuretic family of peptides. The encoded peptides exhibit vasorelaxation activity in laboratory animals and elevated levels of CNP-22 have been observed in the plasma of chronic heart failure patients. [provided by RefSeq, Oct 2015]

Uniprot Description

Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3 ().