Human Troponin C Type 1, Slow (TNNC1) ELISA Kit | TNNC1 elisa kit

Human Troponin C Type 1, Slow (TNNC1) ELISA Kit

Cat. Num. AAA2024717

• Gene Names: TNNC1; TNC; TN-C; TNNC; CMD1Z; CMH13
• Reactivity: Human
• Synonyms: Troponin C Type 1; Slow (TNNC1); Human Troponin C Type 1; Slow (TNNC1) ELISA Kit; TNC; TroponinC; Cardiac; Troponin C; slow skeletal and cardiac muscles; TNNC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Troponin C Type 1, Slow (TNNC1).; No significant cross-reactivity or interference between Troponin C Type 1, Slow (TNNC1) and analogues was observed.
• Sequence Length: 161

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 62.50-4000pg/mL
• Sensitivity: < 27.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin C Type 1, Slow (TNNC1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin C Type 1, Slow (TNNC1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNNC1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin C Type 1, Slow (TNNC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin C Type 1, Slow (TNNC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin C Type 1, Slow (TNNC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin C Type 1, Slow (TNNC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TNNC1 elisa kit

Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 20987725
• NCBI GeneID: 7134
• Molecular Weight: 18,403 Da
• NCBI Official Full Name: Troponin C type 1 (slow)
• NCBI Official Synonym Full Names: troponin C type 1 (slow)
• NCBI Official Symbol: TNNC1
• NCBI Official Synonym Symbols: TNC; TN-C; TNNC; CMD1Z; CMH13
• NCBI Protein Information: troponin C, slow skeletal and cardiac muscles
• UniProt Protein Name: Troponin C, slow skeletal and cardiac muscles
• Protein Family: Troponin
• UniProt Gene Name: TNNC1
• UniProt Synonym Gene Names: TNNC; TN-C
• UniProt Entry Name: TNNC1_HUMAN

NCBI Description

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]

Uniprot Description

TNNC1: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13). A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the troponin C family.

Protein type: Motility/polarity/chemotaxis; Calcium-binding

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; troponin complex; mitochondrion; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; troponin T binding; protein binding; protein homodimerization activity; troponin I binding; calcium ion binding; calcium-dependent protein binding

Biological Process: regulation of ATPase activity; response to metal ion; regulation of muscle contraction; diaphragm contraction; ventricular cardiac muscle morphogenesis; regulation of muscle filament sliding speed; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1z; Cardiomyopathy, Familial Hypertrophic, 13