Rabbit Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody

Cyclin B1 Rabbit pAb

Cat. Num. AAA8808356

• Gene Names: CCNB1; CCNB
• Reactivity: Human, Rat, Mouse
• Synonyms: Cyclin B1; Polyclonal Antibody; Cyclin B1 Rabbit pAb; CCNB1 antibody; G2 mitotic specific cyclin B1 antibody; anti-CCNB1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: PBS with 0.02% sodium azide and 50% glycerol pH7.4.
• Concentration: 1mg/ml (varies by lot)

• Species: Human
• Immunogen: Recombinant Protein
• Preparation and Storage: Store at -20 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of 1) Jurkat, 2) Hela, 3) C2C12, 4) Rat Liver Tissue with Cyclin B1 Rabbit pAb EA244 diluted at 1:2,000.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Tonsil Carcinoma using  Cyclin B1 (EA244)Rabbit pAb diluted at 1:500.)

Related Product Information for anti-CCNB1 antibody

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase of the cell cycle.

NCBI and Uniprot Product Information

• NCBI GI #: 14327896
• NCBI GeneID: 891
• NCBI Accession #: NP_114172.1
• NCBI GenBank Nucleotide #: NM_031966.3
• UniProt Accession #: P14635
• Molecular Weight: 48,337 Da
• NCBI Official Full Name: G2/mitotic-specific cyclin-B1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1
• UniProt Protein Name: G2/mitotic-specific cyclin-B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB1
• UniProt Synonym Gene Names: CCNB
• UniProt Entry Name: CCNB1_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; patched binding; histone kinase activity; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; tissue regeneration; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to mechanical stimulus; cell division; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle