Typical Testing Data/Standard Curve (for reference only)
Rat ARNT2 ELISA Kit | ARNT2 elisa kit
Rat ARNT2 (Aryl Hydrocarbon Receptor Nuclear Translocator 2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ARNT2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ARNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ARNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ARNT2. You can calculate the concentration of Rat ARNT2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting additional roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively. [provided by RefSeq, Dec 2013]
Uniprot Description
ARNT2: Specifically recognizes the xenobiotic response element (XRE). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: transcription factor complex; cytoplasm; nucleus
Molecular Function: signal transducer activity; DNA binding; aryl hydrocarbon receptor binding; protein heterodimerization activity; transcription factor activity
Biological Process: central nervous system development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; brain development; signal transduction; response to estradiol stimulus; negative regulation of apoptosis
Disease: Webb-dattani Syndrome
Research Articles on ARNT2
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Product Notes
The Rat ARNT2 arnt2 (Catalog #AAA2501574) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2501574 ELISA Kit recognizes Rat ARNT2. It is sometimes possible for the material contained within the vial of "ARNT2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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