Typical Testing Data/Standard Curve (for reference only)
Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit | ALOX5 elisa kit
Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit
Gene Names
Alox5; 5LO; 5LX; 5-LO; 5-LOX; AI850497; F730011J02
Reactivity
Rat
Synonyms
Arachidonate-5-Lipoxygenase (ALOX5); Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit; LOX5; 5-LOX; ALOX-5; 5-LO; 5LPG; LOG5; Lipoxygenase 5; ALOX5 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Arachidonate-5-Lipoxygenase (ALOX5).
No significant cross-reactivity or interference between Arachidonate-5-Lipoxygenase (ALOX5) and analogues was observed.
No significant cross-reactivity or interference between Arachidonate-5-Lipoxygenase (ALOX5) and analogues was observed.
Sequence Length
674
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.78-50ng/mL
Sensitivity
< 0.27ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Arachidonate-5-Lipoxygenase (ALOX5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Arachidonate-5-Lipoxygenase (ALOX5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ALOX5 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-5-Lipoxygenase (ALOX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-5-Lipoxygenase (ALOX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-5-Lipoxygenase (ALOX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-5-Lipoxygenase (ALOX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ALOX5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,967 Da
NCBI Official Full Name
arachidonate 5-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase
NCBI Official Symbol
Alox5
NCBI Official Synonym Symbols
5LO; 5LX; 5-LO; 5-LOX; AI850497; F730011J02
NCBI Protein Information
arachidonate 5-lipoxygenase
UniProt Protein Name
Arachidonate 5-lipoxygenase
Protein Family
UniProt Gene Name
Alox5
UniProt Synonym Gene Names
5-LO; 5-lipoxygenase
UniProt Entry Name
LOX5_MOUSE
Uniprot Description
5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.
Protein type: Oxidoreductase; EC 1.13.11.34; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; Nuclear envelope
Cellular Component: extracellular space; nuclear membrane; nuclear matrix; membrane; nuclear envelope lumen; cytoplasm; dendrite; nuclear envelope; nucleus; cytosol; sarcolemma
Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; dioxygenase activity; iron ion binding; metal ion binding; arachidonate 5-lipoxygenase activity; oxidoreductase activity
Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; positive regulation of vasoconstriction; arachidonic acid metabolic process; leukotriene metabolic process; inflammatory response; sensory perception of pain; acute inflammatory response
Research Articles on ALOX5
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Product Notes
The Rat ALOX5 alox5 (Catalog #AAA2020678) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020678 ELISA Kit recognizes Rat ALOX5. It is sometimes possible for the material contained within the vial of "Arachidonate-5-Lipoxygenase (ALOX5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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