Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Apoptosis inducing factor (AIF) Standard Curve.)
Rat Apoptosis Inducing Factor (AIF) ELISA Kit | AIF elisa kit
Rat Apoptosis Inducing Factor (AIF) ELISA Kit
Gene Names
Aifm1; Aif; Pdcd8
Reactivity
Rat
Synonyms
Apoptosis Inducing Factor (AIF); Rat Apoptosis Inducing Factor (AIF) ELISA Kit; AIFM1; RP3-438D16.2; AIF; MGC111425; PDCD8; programmed cell death 8; programmed cell death 8 (apoptosis-inducing factor); striatal apoptosis-inducing factor; AIF elisa kit
Reactivity
Rat
Specificity
Rat Apoptosis inducing factor (AIF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat AIFM1. No significant cross-reactivity or interference between Rat AIFM1 and analogues was observed.
Sequence Length
612
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Apoptosis inducing factor (AIF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Apoptosis inducing factor (AIF) Standard Curve.)
Related Product Information for AIF elisa kit
Description: This Rat Apoptosis inducing factor (AIF) ELISA Kit employs a two-site sandwich ELISA to quantitate AIFM1 in samples. An antibody specific for AIFM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AIFM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AIFM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIFM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Apoptosis inducing factor is involved in initiating a caspase-independent pathway of apoptosis (positive intrinsic regulator of apoptosis) by causing DNA fragmentation and chromatin condensation. It also acts as an NADH oxidase. Another AIF function is to regulate the permeability of the mitochondrial membrane upon apoptosis. Normally it is found behind the outer membrane of the mitochondria and is therefore secluded from the nucleus. However, when the mitochondria is damaged, it moves to the cytosol and to the nucleus. Inactivation of AIF leads to resistance of embryonic stem cells to death following the withdrawal of growth factors indicating that it is involved in apoptosis.
Background: Apoptosis inducing factor is involved in initiating a caspase-independent pathway of apoptosis (positive intrinsic regulator of apoptosis) by causing DNA fragmentation and chromatin condensation. It also acts as an NADH oxidase. Another AIF function is to regulate the permeability of the mitochondrial membrane upon apoptosis. Normally it is found behind the outer membrane of the mitochondria and is therefore secluded from the nucleus. However, when the mitochondria is damaged, it moves to the cytosol and to the nucleus. Inactivation of AIF leads to resistance of embryonic stem cells to death following the withdrawal of growth factors indicating that it is involved in apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
apoptosis-inducing factor 1, mitochondrial
NCBI Official Synonym Full Names
apoptosis inducing factor, mitochondria associated 1
NCBI Official Symbol
Aifm1
NCBI Official Synonym Symbols
Aif; Pdcd8
NCBI Protein Information
apoptosis-inducing factor 1, mitochondrial
UniProt Protein Name
Apoptosis-inducing factor 1, mitochondrial
Protein Family
UniProt Gene Name
Aifm1
UniProt Synonym Gene Names
Aif; Pdcd8
UniProt Entry Name
AIFM1_RAT
NCBI Description
may facilitate apoptotic chromatin condensation and DNA degradation [RGD, Feb 2006]
Research Articles on AIF
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Product Notes
The Rat AIF aifm1 (Catalog #AAA9711878) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711878 ELISA Kit recognizes Rat AIF. It is sometimes possible for the material contained within the vial of "Apoptosis Inducing Factor (AIF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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