Rat Apoptosis Inducing Factor (AIF) ELISA Kit | AIF elisa kit

Rat Apoptosis Inducing Factor (AIF) ELISA Kit

Cat. Num. AAA453735

• Gene Names: Aifm1; Hq; AIF; Pdcd8; AIFsh2
• Reactivity: Rat
• Synonyms: Apoptosis Inducing Factor (AIF); Rat Apoptosis Inducing Factor (AIF) ELISA Kit; PDCD8; Programmed Cell Death protein 8; Apoptosis-inducing factor 1; mitochondrial; AIF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of AIF. No significant cross-reactivity or interference between AIF and analogues was observed.
• Sequence Length: 608

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AIF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AIF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AIF elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AIF in rat serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AIF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 594150372
• NCBI GeneID: 26926
• NCBI Accession #: NP_001277293.1
• NCBI GenBank Nucleotide #: NM_001290364.1
• UniProt Accession #: Q9Z0X1
• Molecular Weight: 66,765 Da
• NCBI Official Full Name: apoptosis-inducing factor 1, mitochondrial isoform 2
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated 1
• NCBI Official Symbol: Aifm1
• NCBI Official Synonym Symbols: Hq; AIF; Pdcd8; AIFsh2
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• Protein Family: Putative apoptosis-inducing factor
• UniProt Gene Name: Aifm1
• UniProt Synonym Gene Names: Aif; Pdcd8

Uniprot Description

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis,and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner ().