Rat Apolipoprotein A1 (Apo A1) ELISA Kit | Apo A1 elisa kit

Rat Apolipoprotein A1 (Apo A1) ELISA Kit

Cat. Num. AAA262568

• Reactivity: Rat
• Synonyms: Apolipoprotein A1 (Apo A1); Rat Apolipoprotein A1 (Apo A1) ELISA Kit; Apo A1 elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 67

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: Up to 0.5 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for Apo A1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Apo A1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4960066
• NCBI GeneID: 335
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A1, partial
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Protein Information: apolipoprotein A-I; apo-AI
• UniProt Protein Name: Apolipoprotein A-I
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Endoplasmic reticulum; Vesicle; Lipid-binding; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; endoplasmic reticulum lumen; early endosome; plasma membrane; extracellular region; cytoplasmic vesicle; cytosol; nucleus; vesicle

Molecular Function: identical protein binding; lipase inhibitor activity; beta-amyloid binding; cholesterol binding; phosphatidylcholine binding; high-density lipoprotein binding; protein binding; enzyme binding; phospholipid transporter activity; chemorepellent activity; cholesterol transporter activity; phospholipid binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding

Biological Process: phototransduction, visible light; negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; sequestering of lipid; negative regulation of interleukin-1 beta secretion; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; response to drug; platelet activation; cholesterol metabolic process; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; lipoprotein biosynthetic process; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood vessel endothelial cell migration; cellular lipid metabolic process; platelet degranulation; phospholipid efflux; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; positive regulation of fatty acid biosynthetic process; receptor-mediated endocytosis; regulation of protein amino acid phosphorylation; cholesterol transport; protein amino acid oxidation; negative regulation of heterotypic cell-cell adhesion; protein stabilization; neurite regeneration; cholesterol efflux; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; reverse cholesterol transport; negative regulation of inflammatory response; endothelial cell proliferation; blood coagulation

Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral