Rat Angiotensin II Receptor Associated Protein (AGTRAP) ELISA Kit | AGTRAP elisa kit

Rat Angiotensin II Receptor Associated Protein (AGTRAP) ELISA Kit

Cat. Num. AAA2122035

• Gene Names: AGTRAP; ATRAP
• Reactivity: Rat
• Synonyms: Angiotensin II Receptor Associated Protein (AGTRAP); Rat Angiotensin II Receptor Associated Protein (AGTRAP) ELISA Kit; ATRAP; Type-1 angiotensin II receptor-associated protein; AT1 receptor-associated protein; AGTRAP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Angiotensin II Receptor Associated Protein (AGTRAP).; No significant cross-reactivity or interference between Angiotensin II Receptor Associated Protein (AGTRAP) and analogues was observed.
• Sequence Length: 152

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Dectection Range: 78-5,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 31pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II Receptor Associated Protein (AGTRAP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II Receptor Associated Protein (AGTRAP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGTRAP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin II Receptor Associated Protein (AGTRAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin II Receptor Associated Protein (AGTRAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin II Receptor Associated Protein (AGTRAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiotensin II Receptor Associated Protein (AGTRAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AGTRAP elisa kit

Signal Transduction; Metabolic Pathway; Endocrinology; Cardiovascular Biology; Hormone Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 16878260
• NCBI GeneID: 57085
• NCBI Official Full Name: Angiotensin II receptor-associated protein
• NCBI Official Synonym Full Names: angiotensin II receptor associated protein
• NCBI Official Symbol: AGTRAP
• NCBI Official Synonym Symbols: ATRAP
• NCBI Protein Information: type-1 angiotensin II receptor-associated protein
• Protein Family: Type-1 angiotensin II receptor-associated protein

NCBI Description

This gene encodes a transmembrane protein localized to the plasma membrane and perinuclear vesicular structures. The gene product interacts with the angiotensin II type I receptor and negatively regulates angiotensin II signaling. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]