Rat Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit | ANGPTL4 elisa kit

Rat Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit

Cat. Num. AAA2021197

• Reactivity: Rat
• Synonyms: Angiopoietin Like Protein 4 (ANGPTL4); Rat Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit; ARP4; FIAF; HFARP; NL2; PGAR; Pp1158; Fasting-Induced Adipose Factor; Hepatic Angiopoietin-Related Protein; Hepatic Fibrinogen/Angiopoietin-Related Protein; ANGPTL4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 4 (ANGPTL4).; No significant cross-reactivity or interference between Angiopoietin Like Protein 4 (ANGPTL4) and analogues was observed.
• Sequence Length: 405

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.57ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPTL4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 4 (ANGPTL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 4 (ANGPTL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 4 (ANGPTL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 4 (ANGPTL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ANGPTL4 elisa kit

Tumor immunity; Endocrinology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 40018598
• NCBI GeneID: 362850
• NCBI Accession #: NP_954546.1
• NCBI GenBank Nucleotide #: NM_199115.2
• UniProt Accession #: Q6TMA8
• Molecular Weight: 44,951 Da
• NCBI Official Full Name: angiopoietin-related protein 4
• NCBI Official Synonym Full Names: angiopoietin-like 4
• NCBI Official Symbol: Angptl4
• NCBI Protein Information: angiopoietin-related protein 4
• UniProt Protein Name: Angiopoietin-related protein 4
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: Angptl4
• UniProt Synonym Gene Names: HFARP
• UniProt Entry Name: ANGL4_RAT

NCBI Description

a circulating protein which causes an increase in plasma very low density lipoprotein by inhibition of lipoprotein lipase activity [RGD, Feb 2006]

Uniprot Description

Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation ().