Typical Testing Data/Standard Curve (for reference only)
Rat ADORA1 ELISA Kit | ADORA1 elisa kit
Rat ADORA1(Adenosine A1 Receptor)ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADORA1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADORA1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADORA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADORA1.You can calculate the concentration of ADORA1in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: presynaptic membrane; asymmetric synapse; postsynaptic membrane; cell soma; integral to plasma membrane; basolateral plasma membrane; endoplasmic reticulum; postsynaptic density; plasma membrane; dendritic spine; presynaptic active zone; terminal button; axolemma
Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; adenosine receptor activity, G-protein coupled; purine nucleoside binding; phospholipase C activity; heterotrimeric G-protein binding
Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; negative regulation of glutamate secretion; activation of MAPKK activity; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; neurological control of breathing; positive regulation of peptide secretion; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; cell-cell signaling; positive regulation of nucleoside transport; positive regulation of epidermal growth factor receptor activity; negative regulation of vasodilation; negative regulation of lipid catabolic process; negative regulation of blood pressure; negative regulation of synaptic transmission, GABAergic; protein targeting to membrane; detection of temperature stimulus involved in sensory perception of pain; inflammatory response; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of acute inflammatory response; negative regulation of synaptic transmission, glutamatergic; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of sensory perception of pain; cognition; regulation of excitatory postsynaptic membrane potential; lipid catabolic process; positive regulation of blood pressure; negative regulation of apoptosis
Research Articles on ADORA1
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Product Notes
The Rat ADORA1 adora1 (Catalog #AAA2515905) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515905 ELISA Kit recognizes Rat ADORA1. It is sometimes possible for the material contained within the vial of "ADORA1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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