Human Phosphodiesterase 9A (PDE9A) ELISA Kit | PDE9A elisa kit

Human Phosphodiesterase 9A (PDE9A) ELISA Kit

Cat. Num. AAA166918

• Gene Names: PDE9A; HSPDE9A2
• Reactivity: Human
• Synonyms: Phosphodiesterase 9A (PDE9A); Human Phosphodiesterase 9A (PDE9A) ELISA Kit; PDE9A elisa kit

• Reactivity: Human
• Sequence Length: 533

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.75-48ng/ml
• Sensitivity: 0.36ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDE9A elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Phosphodiesterase 9A (also known as PDE9A) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PDE9A antibody. PDE9A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PDE9A Antibody is added and binds to PDE9A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PDE9A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PDE9A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 14290551
• NCBI GeneID: 5152
• Molecular Weight: 61,709 Da
• NCBI Official Full Name: Phosphodiesterase 9A
• NCBI Official Synonym Full Names: phosphodiesterase 9A
• NCBI Official Symbol: PDE9A
• NCBI Official Synonym Symbols: HSPDE9A2
• NCBI Protein Information: high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
• UniProt Protein Name: High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
• Protein Family: High affinity cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE9A

NCBI Description

The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed:9624146, PubMed:18757755, PubMed:21483814). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory ().