Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1) Standard Curve.)
Rat 5-Aminolevulinate Synthase, Nonspecific, Mitochondrial (ALAS1) ELISA Kit | ALAS1 elisa kit
Rat 5-Aminolevulinate Synthase, Nonspecific, Mitochondrial (ALAS1) ELISA Kit
Gene Names
Alas1; ALAS
Reactivity
Rat
Synonyms
5-Aminolevulinate Synthase; Nonspecific; Mitochondrial (ALAS1); Rat 5-Aminolevulinate Synthase; Mitochondrial (ALAS1) ELISA Kit; ALAS1; ALAS; ALAS3; ALASH; MIG4; 5-aminolevulinate synthase 1; migration-inducing protein 4; ALAS1 elisa kit
Reactivity
Rat
Specificity
Rat 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ALAS1. No significant cross-reactivity or interference between Rat ALAS1 and analogues was observed.
Sequence Length
642
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1) Standard Curve.)
Related Product Information for ALAS1 elisa kit
Description: This Rat 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1) ELISA Kit employs a two-site sandwich ELISA to quantitate ALAS1 in samples. An antibody specific for ALAS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALAS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALAS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALAS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Delta-aminolevulinate synthase 1 is an aminolevulinic acid synthase. Delta-aminolevulinate synthase catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2. ALAS1encodes a housekeeping form of ALAS inasmuch as it is expressed in all cell types, including erythroid cells, whereas ALAS2 encodes a red cell-specific form. ALAS1 and ALAS2 have only 59% identity in amino acid sequence; there was no homology in the amino-terminal region and about 73% homology after hepatic residue 197.
Background: Delta-aminolevulinate synthase 1 is an aminolevulinic acid synthase. Delta-aminolevulinate synthase catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2. ALAS1encodes a housekeeping form of ALAS inasmuch as it is expressed in all cell types, including erythroid cells, whereas ALAS2 encodes a red cell-specific form. ALAS1 and ALAS2 have only 59% identity in amino acid sequence; there was no homology in the amino-terminal region and about 73% homology after hepatic residue 197.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
5-aminolevulinate synthase, nonspecific, mitochondrial
NCBI Official Synonym Full Names
5'-aminolevulinate synthase 1
NCBI Official Symbol
Alas1
NCBI Official Synonym Symbols
ALAS
NCBI Protein Information
5-aminolevulinate synthase, nonspecific, mitochondrial
UniProt Protein Name
5-aminolevulinate synthase, nonspecific, mitochondrial
Protein Family
UniProt Gene Name
Alas1
UniProt Synonym Gene Names
ALAS-H
Uniprot Description
MiscellaneousThere are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.
Research Articles on ALAS1
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Product Notes
The Rat ALAS1 alas1 (Catalog #AAA9711903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711903 ELISA Kit recognizes Rat ALAS1. It is sometimes possible for the material contained within the vial of "5-Aminolevulinate Synthase, Nonspecific, Mitochondrial (ALAS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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