Rat Transferrin (TRF) CLIA Kit | TRF clia kit

Rat Transferrin (TRF) CLIA Kit

Cat. Num. AAA2025460

• Gene Names: TF; TFQTL1; PRO1557; PRO2086
• Reactivity: Rat
• Synonyms: Transferrin (TRF); Rat Transferrin (TRF) CLIA Kit; TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin; TRF clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transferrin (TRF).; No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.
• Sequence Length: 698

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 54.9-40,000pg/mL
• Sensitivity: < 20.3pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin (TRF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin (TRF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRF clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transferrin (TRF) level in the sample or standard.;

Product Categories/Family for TRF clia kit

Tumor immunity; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 339453
• NCBI GeneID: 7018
• Molecular Weight: 77,064 Da
• NCBI Official Full Name: transferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: TF
• NCBI Official Synonym Symbols: TFQTL1; PRO1557; PRO2086
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• Protein Family: TBP-related factor
• UniProt Gene Name: TF
• UniProt Synonym Gene Names: Transferrin
• UniProt Entry Name: TRFE_HUMAN

NCBI Description

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Uniprot Description

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: extracellular space; recycling endosome; cell surface; perinuclear region of cytoplasm; endocytic vesicle; apical plasma membrane; late endosome; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; basal plasma membrane; endosome membrane; coated pit; vesicle

Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding

Biological Process: retinal homeostasis; platelet activation; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport

Disease: Atransferrinemia