Typical Testing Data/Standard Curve (for reference only)
Mouse TRF (Transferrin) CLIA Kit | TRF clia kit
Mouse TRF (Transferrin) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to TRF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TRF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TRF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of TRF. You can calculate the concentration of TRF in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]
Uniprot Description
TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q22.1
Cellular Component: extracellular space; cell surface; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; coated pit; recycling endosome; extrinsic to external side of plasma membrane; perinuclear region of cytoplasm; endocytic vesicle; late endosome; apical plasma membrane; basal plasma membrane; endosome membrane; vesicle
Molecular Function: protein binding; ferric iron binding; ferrous iron binding; ferric iron transmembrane transporter activity
Biological Process: retinal homeostasis; platelet activation; iron ion homeostasis; platelet degranulation; cellular iron ion homeostasis; transferrin transport; regulation of protein stability; blood coagulation; transmembrane transport; positive regulation of receptor-mediated endocytosis
Disease: Atransferrinemia
Research Articles on TRF
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Product Notes
The Mouse TRF tf (Catalog #AAA2531857) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531857 CLIA Kit recognizes Mouse TRF. It is sometimes possible for the material contained within the vial of "TRF (Transferrin), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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