Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) CLIA Kit | MIP3a clia kit

Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) CLIA Kit

Cat. Num. AAA2025442

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• Reactivity: Rat
• Synonyms: Macrophage Inflammatory Protein 3 Alpha (MIP3a); Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) CLIA Kit; CCL20; CKb4; LARC; MIP3-A; SCYA20; ST38; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20; Beta-Chemokine Exodus-1; Liver And Activation-Regulated Chemokine; MIP3a clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Alpha (MIP3a).; No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Alpha (MIP3a) and analogues was observed.
• Sequence Length: 96

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: < 0.53pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP3a clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Inflammatory Protein 3 Alpha (MIP3a) level in the sample or standard.;

Product Categories/Family for MIP3a clia kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 1790925
• NCBI GeneID: 6364
• Molecular Weight: 10,691 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 alpha
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• NCBI Protein Information: C-C motif chemokine 20
• UniProt Protein Name: C-C motif chemokine 20
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20; MIP-3-alpha
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; wound healing; defense response to bacterium; immune response; chemotaxis; inflammatory response; positive regulation of interleukin-1 alpha biosynthetic process; signal transduction; positive regulation of nitric-oxide synthase biosynthetic process