Rat Interleukin 17F (IL17F) CLIA Kit | IL17F clia kit

Rat Interleukin 17F (IL17F) CLIA Kit

Cat. Num. AAA2709014

• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Interleukin 17F (IL17F); Rat Interleukin 17F (IL17F) CLIA Kit; Interleukin 17F; ML-1; IL17F clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 17F (IL17F).; No significant cross-reactivity or interference between Interleukin 17F (IL17F) and analogues was observed.
• Sequence Length: 153

• Applicable Applications for IL17F clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.64pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17F (IL17F) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17F (IL17F) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 17F (IL17F). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 17F (IL17F). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 17F (IL17F) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 62543517
• NCBI Accession #: NP_001015011.1
• NCBI GenBank Nucleotide #: NP_001015011.1
• UniProt Accession #: Q5BJ95
• NCBI Official Full Name: interleukin-17F
• UniProt Protein Name: Interleukin-17F
• Protein Family: Interleukin
• UniProt Gene Name: Il17f
• UniProt Synonym Gene Names: IL-17F
• UniProt Entry Name: IL17F_RAT

Uniprot Description

IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Cellular Component: extracellular space

Molecular Function: protein homodimerization activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine binding; cytokine activity

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; regulation of transforming growth factor beta receptor signaling pathway; regulation of interleukin-8 biosynthetic process; lymphotoxin A biosynthetic process; cartilage development; regulation of interleukin-2 biosynthetic process; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; cytokine biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response