Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit | GM-CSF clia kit

Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit

Cat. Num. AAA2532249

• Gene Names: CSF2; GMCSF
• Reactivity: Rat
• Synonyms: GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor); Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit; GMCSF; CSF2; GM-CSF clia kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant GM-CSF. No significant cross-reactivity or interference between GM-CSF and analogues was observed.
• Sequence Length: 144

• Assay Type: Sandwich
• Detection Range: 7.813-500pg/mL
• Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GM-CSF clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of GM-CSF concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GM-CSF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GM-CSF. You can calculate the concentration of GM-CSF in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_000749.2
• NCBI GenBank Nucleotide #: NM_000758.3
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of cytolysis; axon guidance; epithelial fluid transport; embryonic placenta development; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; positive regulation of interleukin-23 production; MAPKKK cascade; myeloid dendritic cell differentiation; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; small GTPase mediated signal transduction; Ras protein signal transduction; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication