Rat Bone Morphogenetic Protein 2 (BMP2) CLIA Kit | BMP2 clia kit

Rat Bone Morphogenetic Protein 2 (BMP2) CLIA Kit

Cat. Num. AAA2000895

• Gene Names: BMP2; BDA2; BMP2A
• Reactivity: Rat
• Synonyms: Bone Morphogenetic Protein 2 (BMP2); Rat Bone Morphogenetic Protein 2 (BMP2) CLIA Kit; BMP2A; BMP-2A; Hemochromatosis Modifier; BMP2 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.
• Sequence Length: 396

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 5.49-4,000pg/mL
• Sensitivity: < 2.31pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP2 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 2 (BMP2) level in the sample or standard.;

Product Categories/Family for BMP2 clia kit

Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4557369
• NCBI GeneID: 650
• NCBI Accession #: NP_001191.1
• NCBI GenBank Nucleotide #: NM_001200.2
• UniProt Accession #: P12643
• Molecular Weight: 44,702 Da
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: BMP2
• NCBI Official Synonym Symbols: BDA2; BMP2A
• NCBI Protein Information: bone morphogenetic protein 2
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP2
• UniProt Synonym Gene Names: BMP2A; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

Uniprot Description

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).