Rat A Disintegrin And Metalloprotease 10 (ADAM10) CLIA Kit | ADAM10 clia kit

Rat A Disintegrin And Metalloprotease 10 (ADAM10) CLIA Kit

Cat. Num. AAA2708845

• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: A Disintegrin And Metalloprotease 10 (ADAM10); Rat A Disintegrin And Metalloprotease 10 (ADAM10) CLIA Kit; A Disintegrin And Metalloprotease 10; CD156-C; CD156c; MADM; Kuz; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; ADAM10 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10).; No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.
• Sequence Length: 544

• Applicable Applications for ADAM10 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 6.86-5,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 3.13pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the A Disintegrin And Metalloprotease 10 (ADAM10) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 37096570
• UniProt Accession #: Q10743
• NCBI Official Full Name: Disintegrin and metalloproteinase domain-containing protein 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam10
• UniProt Synonym Gene Names: Madm; ADAM 10
• UniProt Entry Name: ADA10_RAT

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling (By similarity)

Protein type: Motility/polarity/chemotaxis; Vesicle; Cell adhesion; EC 3.4.24.81; Cell surface; Membrane protein, integral; Protease

Cellular Component: cell surface; cytoplasm; focal adhesion; Golgi apparatus; Golgi-associated vesicle; integral to membrane; intracellular membrane-bound organelle; membrane; nucleus; postsynaptic density; trans-Golgi network

Molecular Function: endopeptidase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; protein homodimerization activity; protein kinase binding; SH3 domain binding; zinc ion binding

Biological Process: constitutive protein ectodomain proteolysis; in utero embryonic development; membrane protein ectodomain proteolysis; monocyte activation; negative regulation of cell adhesion; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; protein amino acid phosphorylation; protein processing; regulation of osteoclast differentiation