Rap guanine nucleotide exchange factor 2 (Rapgef2) Recombinant Protein | Rapgef2 recombinant protein

Recombinant Mouse Rap guanine nucleotide exchange factor 2 (Rapgef2) , partial

• Gene Names: Rapgef2; Pdzgef1; nRapGEP; CNRasGEF; RA-GEF-1; mKIAA0313; 5830453M24Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Rap guanine nucleotide exchange factor 2 (Rapgef2); Recombinant Mouse Rap guanine nucleotide exchange factor 2 (Rapgef2); partial; Rapgef2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,496

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 568924419
• NCBI GeneID: 76089
• NCBI Accession #: XP_006502317.1
• NCBI GenBank Nucleotide #: XM_006502254.3
• UniProt Accession #: Q8CHG7; E9QMD0
• Molecular Weight: 166,415 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 2 isoform X5
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 2
• NCBI Official Symbol: Rapgef2
• NCBI Official Synonym Symbols: Pdzgef1; nRapGEP; CNRasGEF; RA-GEF-1; mKIAA0313; 5830453M24Rik
• NCBI Protein Information: rap guanine nucleotide exchange factor 2
• UniProt Protein Name: Rap guanine nucleotide exchange factor 2
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: Rapgef2
• UniProt Synonym Gene Names: Kiaa0313; Pdzgef1; CNrasGEF; nRap GEP; PDZ-GEF1

Uniprot Description

Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.