Human RAP1 GTPase activating protein ELISA Kit | RAP1GAP elisa kit

Human Rap1 GTPase-activating protein 1, RAP1GAP ELISA Kit

Cat. Num. AAA9330527

• Gene Names: RAP1GAP; RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII
• Reactivity: Human
• Synonyms: RAP1 GTPase activating protein; Human Rap1 GTPase-activating protein 1; RAP1GAP ELISA Kit; Human Rap1 GTPase-activating protein 1 (RAP1GAP) ELISA kit; RP11-63N8.1; KIAA0474; RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP; RAP1; GTPase activating protein 1; RAP1GAP elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human RAP1GAP1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated RAP1GAP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RAP1GAP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RAP1GAP1 and RAP1GAP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RAP1GAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for RAP1GAP elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated RAP1GAP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RAP1GAP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RAP1GAP1 and RAP1GAP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RAP1GAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224809573
• NCBI GeneID: 5909
• NCBI Accession #: NP_001139129.1
• NCBI GenBank Nucleotide #: NM_001145657.1
• UniProt Accession #: P47736; O75062; Q5T3S9; Q5T3T4; Q7Z5S8; Q9UQ51; J3QSS6
• Molecular Weight: 73,361 Da
• NCBI Official Full Name: rap1 GTPase-activating protein 1 isoform b
• NCBI Official Synonym Full Names: RAP1 GTPase activating protein
• NCBI Official Symbol: RAP1GAP
• NCBI Official Synonym Symbols: RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII
• NCBI Protein Information: rap1 GTPase-activating protein 1
• UniProt Protein Name: Rap1 GTPase-activating protein 1
• Protein Family: Rap1 GTPase-activating protein
• UniProt Gene Name: RAP1GAP
• UniProt Synonym Gene Names: KIAA0474; RAP1GA1; Rap1GAP; Rap1GAP1
• UniProt Entry Name: RPGP1_HUMAN

NCBI Description

This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011]

Uniprot Description

RAP1GAP: a GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

Protein type: Motility/polarity/chemotaxis; GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: Golgi membrane; membrane; cytosol

Molecular Function: GTPase activity; protein binding; protein homodimerization activity; Ras GTPase binding; GTPase activator activity

Biological Process: metabolic process; signal transduction