Rap 1A recombinant protein

Rap 1A

Cat. Num. AAA396494

• Gene Names: RAP1A; RAP1; KREV1; KREV-1; SMGP21
• Reactivity: Human
• Applications: Affinity Binding
• Synonyms: Rap 1A; Rap 1A Recombinant G-Protein; G protein; Rap 1A recombinant protein

• Reactivity: Human
• Form/Format: Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol
• Sequence Length: 184

• Applicable Applications for Rap 1A recombinant protein: Affinity Binding
• Immunogen: Recombinant GST protein from E. Coli
• Preparation and Storage: Product should be stored at -70 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for Rap 1A recombinant protein

Rap1A is a 21 kDa small GTP-binding protein that antagonizes the Ras transforming activity through tight binding to Ras-GAP. It interferes with Ras-dependent Raf1 activation by inhibiting binding of Ras to the cysteine-rich region of Raf1. This protein is localized in the endoplasmic reticulum, late endosomes, and lysosomes, indicating its potential role in the regulation of intracellular protein degradation. Rap1a contains a C-terminal CLLL motif for post-translational geranylgeranylation and a single site for phosphorylation by protein kinase A. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.

Product Categories/Family for Rap 1A recombinant protein

Recombinant Protein; G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 58331202
• NCBI GeneID: 5906
• NCBI Accession #: NP_001010935.1
• NCBI GenBank Nucleotide #: NM_001010935.1
• UniProt Accession #: P62834; P10113
• Molecular Weight: 20,987 Da
• NCBI Official Full Name: ras-related protein Rap-1A
• NCBI Official Synonym Full Names: RAP1A, member of RAS oncogene family
• NCBI Official Symbol: RAP1A
• NCBI Official Synonym Symbols: RAP1; KREV1; KREV-1; SMGP21
• NCBI Protein Information: ras-related protein Rap-1A; C21KG; G-22K; RAS-related protein RAP1A; Ras-related protein Krev-1; GTP-binding protein smg p21A
• UniProt Protein Name: Ras-related protein Rap-1A
• UniProt Gene Name: RAP1A
• UniProt Synonym Gene Names: KREV1
• UniProt Entry Name: RAP1A_HUMAN

NCBI Description

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes.

Enzyme regulation: Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.

Subunit structure: Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A. Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1. In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3.

Subcellular location: Cell membrane; Lipid-anchor.

Sequence similarities: Belongs to the small GTPase superfamily. Ras family.

Sequence caution: The sequence CAB55685.2 differs from that shown. Reason: Erroneous gene model prediction.