Estrogen Receptor Phospho-Regulation Antibody Sampler Kit | RaK antibody sampler kit
Estrogen Receptor Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation
Synonyms
Estrogen Receptor Phospho-Regulation; Estrogen RaK; RaK antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for RaK antibody sampler kit
Western Blot (WB), Immunocytochemistry (ICC), ELISA (EIA), Immunoprecipitation (IP)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for RaK antibody sampler kit
Kit Summary: The ERalpha Phospho-Regulation antibody sampler kit can be used to detect phosphorylation of Tyr-537 relative to total ERalphaexpression levels. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect phospho-Tyr-537, as wellas a rabbit polyclonal antibody to detect ERalpha. The kit also includes anti-Rabbit Light Chain specific:HRP and anti-MouseIg specific:HRP secondary reagents for detection of antibodies in Western blot, ELISA, or immunocytochemistry.
Background: Estrogen receptor alpha (ERalpha) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2). AF-1 and AF-2 activate transcription independently and synergistically, and act in apromoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERalphaactivity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation functiondomain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERalpha activity. In additionto these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, andtranscriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERalphaand degradation. Thus, a variety of phosphorylation events control ERalpha activity.
Background: Estrogen receptor alpha (ERalpha) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2). AF-1 and AF-2 activate transcription independently and synergistically, and act in apromoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERalphaactivity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation functiondomain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERalpha activity. In additionto these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, andtranscriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERalphaand degradation. Thus, a variety of phosphorylation events control ERalpha activity.
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Product Notes
The Human, Rat, Mouse RaK (Catalog #AAA474312) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474312 Antibody Sampler Kit recognizes Human, Rat, Mouse RaK. AAA Biotech's Estrogen Receptor Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the RaK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen Receptor Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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