Typical Testing Data/Standard Curve (for reference only)
Human Transmembrane protein 173 (TMEM173) ELISA Kit | TMEM173 elisa kit
Human Transmembrane protein 173 (TMEM173) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TMEM173 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TMEM173 antibody and an TMEM173-HRP conjugate. The assay sample and buffer are incubated together with TMEM173-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM173 concentration since TMEM173 from samples and TMEM173-HRP conjugate compete for the anti-TMEM173 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM173 from the sample, fewer sites are left to bind TMEM173-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM173 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Uniprot Description
TMEM173: Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double- stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti- viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Associates with the MHC-II complex. Homodimer; 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization. Interacts with DDX58/RIG-I, MAVS/VISA and SSR2. Interacts with RNF5 and TRIM56. Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta. Ubiquitously expressed. Belongs to the TMEM173 family.
Protein type: Mitochondrial; Endoplasmic reticulum; Membrane protein, integral; Apoptosis; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 5q31.2
Cellular Component: Golgi apparatus; mitochondrial outer membrane; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; peroxisome
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; ubiquitin protein ligase binding; transcription factor binding; protein kinase binding
Biological Process: regulation of interferon type I production; positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; apoptosis; positive regulation of transcription factor import into nucleus; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; activation of innate immune response; defense response to virus; interferon-beta production
Disease: Sting-associated Vasculopathy, Infantile-onset
Research Articles on TMEM173
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Product Notes
The Human TMEM173 tmem173 (Catalog #AAA7237945) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7237945 ELISA Kit recognizes Human TMEM173. It is sometimes possible for the material contained within the vial of "Transmembrane protein 173 (TMEM173), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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