UV excision repair protein RAD23 homolog A (RAD23A) Recombinant Protein | RAD23A recombinant protein

Recombinant Human UV excision repair protein RAD23 homolog A (RAD23A)(T131A)

Cat. Num. AAA9018520

• Gene Names: RAD23A; HR23A; HHR23A
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: UV excision repair protein RAD23 homolog A (RAD23A); Recombinant Human UV excision repair protein RAD23 homolog A (RAD23A)(T131A); UV excision repair protein RAD23 homolog A(HR23A)(hHR23A); RAD23A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-362aa(T131A), Full Length of Isoform 3
• Sequence: MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPATSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE

• Species: Homo sapiens (Human)
• Relevance: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.; Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.; (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Product Categories/Family for RAD23A recombinant protein

Epigenetics and Nuclear Signaling

References

Components of the ubiquitin-proteasome pathway compete for surfaces on Rad23 family proteins.Goh A.M., Walters K.J., Elsasser S., Verma R., Deshaies R.J., Finley D., Howley P.M.BMC Biochem. 9:4-4(2008)

NCBI and Uniprot Product Information

• NCBI GI #: 392996949
• NCBI GeneID: 5886
• NCBI Accession #: NP_001257291.1
• NCBI GenBank Nucleotide #: NM_001270362.1
• UniProt Accession #: P54725
• Molecular Weight: 39,609 Da
• NCBI Official Full Name: UV excision repair protein RAD23 homolog A isoform 2
• NCBI Official Synonym Full Names: RAD23 homolog A (S. cerevisiae)
• NCBI Official Symbol: RAD23A
• NCBI Official Synonym Symbols: HR23A; HHR23A
• NCBI Protein Information: UV excision repair protein RAD23 homolog A; RAD23, yeast homolog, A
• UniProt Protein Name: UV excision repair protein RAD23 homolog A
• Protein Family: UV excision repair protein
• UniProt Gene Name: RAD23A
• UniProt Synonym Gene Names: HR23A; hHR23A
• UniProt Entry Name: RD23A_HUMAN

NCBI Description

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

RAD23A: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Belongs to the RAD23 family.

Protein type: DNA repair, damage; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: proteasome complex; nucleoplasm; cytoplasm

Molecular Function: protein binding; damaged DNA binding; polyubiquitin binding; single-stranded DNA binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of viral genome replication; viral reproduction; nucleotide-excision repair; regulation of proteasomal ubiquitin-dependent protein catabolic process