Cell cycle checkpoint protein RAD17 (Rad17) Recombinant Protein | Rad17 recombinant protein

Recombinant Mouse Cell cycle checkpoint protein RAD17 (Rad17)

Cat. Num. AAA1289039

• Gene Names: Rad17; MmRad24
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Cell cycle checkpoint protein RAD17 (Rad17); Recombinant Mouse Cell cycle checkpoint protein RAD17 (Rad17); Rad17 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-688, Full length protein
• Sequence: MSETFLRPKVSSTKVTDWVAPAFDDFEANTAITTITASSLTFSNSSHRRKYLPSTLESNRLSARKRGRLSLEQTHGLETSRERLSDNEPWVDKYKPETQHELAVHKKKIEEVETWLKAQVLEVKPKQGGSVLLITGPPGCGKTTTIKILSKELGIQVQEWVNPILPDFQKDDYKELLSLESNFSVVPYQSQIAVFNDFLLRATKYSKLQMLGDDLTTDKKIILVEELPNQFYRDPNALHEILRKHVQIGRCPLVFIVSDSVSGDNNQRLLFPRNIQEECSVSNISFNPVAPTIMMKFLNRIVTIEASKNGEKIIVPNKTSLELLCQGCSGDIRSAINSLQFSSSKGENSSWSKKKRMSLKSDAAISKSKQKKKHNSTLENQEIQAIGGKDVSLFLFRALGKILYCKRAPLTELDSPRLPAHLSEHDRDTLLVQPEEIVEMSHMPGDFFNLYLHQNYIDFFAEVDDLVPASEFLSFADILGGDWNTRSLLREYSTSVATRGVMHSNKARGFAHCQGGSSFRPLHKPQWFLIQKKYRENCLAAKALFVDFCLPALCLQTQLLPYLALLTIPMRNKAQISFIQDVGRLPLKRSFGRLKMEALTDRELGLIDPDSGDESPHSGGQPAQEAPGEPAQAAQNADPETWSLPLSQNSGSDLPASQPQPFSSKVDMEEEEEEEEDIIIEDYDSEET
• Sequence Length: 688

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Rad17 recombinant protein

This protein is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 113199779
• NCBI GeneID: 19356
• NCBI Accession #: NP_001037836.1
• NCBI GenBank Nucleotide #: NM_001044371.2
• UniProt Accession #: Q6NXW6; O88934; O89024
• Molecular Weight: 77,391 Da
• NCBI Official Full Name: cell cycle checkpoint protein RAD17 isoform 1
• NCBI Official Synonym Full Names: RAD17 checkpoint clamp loader component
• NCBI Official Symbol: Rad17
• NCBI Official Synonym Symbols: MmRad24
• NCBI Protein Information: cell cycle checkpoint protein RAD17
• UniProt Protein Name: Cell cycle checkpoint protein RAD17
• Protein Family: Checkpoint protein
• UniProt Gene Name: Rad17

Uniprot Description

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination (). Essential for embryonic development. May be involved in homologous recombination.