Monkey Ras-related C3 Botulinum Toxin Substrate 1 ELISA Kit | RAC1 elisa kit

Monkey Ras-related C3 Botulinum Toxin Substrate 1 ELISA Kit

Cat. Num. AAA750101

• Gene Names: Rac1; AL023026; D5Ertd559e
• Reactivity: Monkey
• Synonyms: Ras-related C3 Botulinum Toxin Substrate 1; Monkey Ras-related C3 Botulinum Toxin Substrate 1 ELISA Kit; RAC1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This Rac1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Rac1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This Rac1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Rac1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Related Product Information for RAC1 elisa kit

Principle of the Assay: Rac1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Rac1 antibody and an Rac1-HRP conjugate. The assay sample and buffer are incubated together with Rac1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Rac1 concentration since Rac1 from samples and Rac1-HRP conjugate compete for the anti-Rac1 antibody binding site. Since the number of sites is limited, as more sites are occupied by Rac1 from the sample, fewer sites are left to bind Rac1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Rac1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45592934
• NCBI GeneID: 19353
• NCBI Accession #: NP_033033.1
• NCBI GenBank Nucleotide #: NM_009007.2
• Molecular Weight: 21,450 Da
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 1
• NCBI Official Synonym Full Names: RAS-related C3 botulinum substrate 1
• NCBI Official Symbol: Rac1
• NCBI Official Synonym Symbols: AL023026; D5Ertd559e
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1; p21-Rac1
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• Protein Family: Ras-related protein
• UniProt Gene Name: Rac1
• UniProt Entry Name: RAC1_MOUSE

Uniprot Description

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: G protein, monomeric, Rho; G protein; Motility/polarity/chemotaxis; G protein, monomeric

Cellular Component: extrinsic to plasma membrane; focal adhesion; cytoplasmic membrane-bound vesicle; actin filament; trans-Golgi network; cytosol; Golgi membrane; cell projection; membrane; lamellipodium; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; phagocytic cup

Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; Rho GDP-dissociation inhibitor binding; histone deacetylase binding; thioesterase binding; nucleotide binding; protein kinase binding; Rab GTPase binding

Biological Process: axon guidance; metabolic process; cell motility involved in cell locomotion; regulation of neuron maturation; chemotaxis; regulation of cell migration; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; small GTPase mediated signal transduction; positive regulation of stress fiber formation; dendrite development; regulation of cell morphogenesis; cell adhesion; bone resorption; lamellipodium biogenesis; embryonic olfactory bulb interneuron precursor migration; intercellular junction assembly and maintenance; cell migration; mast cell chemotaxis; Wnt receptor signaling pathway, planar cell polarity pathway; dendrite morphogenesis; synaptic transmission, GABAergic; positive regulation of phosphoinositide 3-kinase activity; actin filament organization; cerebral cortex GABAergic interneuron development; endocytosis; cytoskeleton organization and biogenesis; engulfment of apoptotic cell; G-protein coupled receptor protein signaling pathway; cell proliferation; negative regulation of interleukin-23 production; hyperosmotic response; positive regulation of actin filament polymerization; homeostasis of number of cells within a tissue; organization of an anatomical structure; auditory receptor cell morphogenesis; ruffle organization and biogenesis; cerebral cortex radially oriented cell migration; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; actin cytoskeleton organization and biogenesis; positive regulation of DNA replication