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Pyruvate Kinase Active Protein | PKM active protein

Pyruvate Kinase (PK), Rabbit Muscle

Purity
Purified
Synonyms
Pyruvate Kinase; Pyruvate Kinase (PK); Rabbit Muscle; ATP:Pyruvate 2-0-phosphotransferase; PK; >250 U/mg; PKM active protein
Ordering
For Research Use Only!
Host
Rabbit skeletal muscle
Purity/Purification
Purified
Form/Format
Powder, lyophilized from tris chloride, pH 7.7
Sequence Length
477
Application Notes
Application: Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes.
EC Number
2.7.1.40
CAS Number
9001-59-6
Appearance
White to off white powder.
Unit Definition
One unit will catalyze the conversion of one micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.6 and 37 °C.
Activity
170 U/mg
Protein
1.1 mg protein/mg (E0.1%280nm = 0.54)
Specific Activity
150 U/mg protein
Contaminants
CPK: <1.0%
LDH: <0.01%
Reconstitution
Reconstitute with 0.9% saline. Prepare further dilutions in tris buffered saline with 1% BSA. Aliquot and freeze at -20°C after reconstitution.
Handling
Handle as a potentially hazardous substance.
Preparation and Storage
Store at -20°C
Protect from moisture
Related Product Information for PKM active protein
Custom preparations, technical support, bulk quantities and aliquoting available.

Pyruvate Kinase is an enzyme involved in glycolysis. Pyruvate Kinase (PK) catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding a pyruvate molecule and producing one molecule ATP.
Product Categories/Family for PKM active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
pyruvate kinase
NCBI Official Symbol
pyk
NCBI Protein Information
pyruvate kinase
Protein Family

Similar Products

Product Notes

The PKM (Catalog #AAA173183) is an Active Protein produced from Rabbit skeletal muscle and is intended for research purposes only. The product is available for immediate purchase. Application: Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes. Researchers should empirically determine the suitability of the PKM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Kinase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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