Rabbit anti-Rat, Mouse ADCY5 Antibody | anti-ADCY5 antibody

ADCY5 Antibody

Cat. Num. AAA540324

• Reactivity: Rat, Mouse
• Applications: Dot Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: ADCY5; ADCY5 Antibody; anti-ADCY5 antibody

• Host: Rabbit
• Reactivity: Rat, Mouse
• Form/Format: Affinity Purified Immunoglobulins

• Applicable Applications for anti-ADCY5 antibody: Confocal Microscopy, Dot Blot (DB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: DB: 1:10,000; ELISA: 1:10,000; ICC: 1:200-1:1,000; IF: 1:200-1:1,000; IHC: 1:200-1:1,000; IP: 1:200; WB: 1:500
• Immunogen: Synthetic peptide corresponding to unique amino acid sequence on rat Particulate Adenylyl Cyclase 5 protein.
• Preparation and Storage: Store at -20°C for long term storage.

Western Blot (WB)

Testing Data

Testing Data

(WB of MBS540324 with MBS543025. 1 :500 antibody dilution in DiluObuffer. Apparent MW is 135 kDa )

Related Product Information for anti-ADCY5 antibody

The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G prolein-coupled receptors. Nine human AC isoforms (AC-1 through AC-9), encoded by separate genes, have been identified up to now (1). Most of the adenylate cyclase genes are comprise of 11-26 exons and distributed over a q6-430 kb. Majority of the adenylate cyclases previously described are expressed discretely in defined peripheral tissues, the type 4 adenylate cyclase (AC4) is apparenlly expressed in a variety of peripheral tissues and in the central nervous system, mainly in olfactory system. AC-5 is localized on photoreceptor cells and are also designated as GCl and GC2. The AC-S protein resembles the other adenylyl cyclases in its predicted and proposed structure. AC-5 resembles the type 6 (AC-6) adenylyl cyclase in its amino acid sequence but becomes divergent at N and C-terminal ends. The AC5 and AC6 proteins are colocalized in most of the visual organs (photoreceptor cells) are associated with other protein complexes. Both AC5 and AC6 enzymes play an important role in synaptic plasticity by coordinating overlapping synaptic inputs from Gs and Gi coupled receptor stimulation (3).

The Anti-AC5-seleclive antibodies were generated against conserved sequences alar near the C-Ierminal end of the protein that are unique to AC5-protein. The AC5-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The AC4 antibodies label a 127-130 kDa protein in Westem blot positive control and in rat ol factory bUlb. Anti-AC5-selective antibodies are also available in affinity-purified form for confocal, WB and IHC analyses.

Product Categories/Family for anti-ADCY5 antibody

Primary Antibodies; Signal Transduction; Second Messengers; cAMP; ADCY5 Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 1758331
• NCBI GeneID: 64532
• UniProt Accession #: Q04400
• NCBI Official Full Name: Rattus norvegicus adenylyl cyclase type V mRNA, complete cds
• NCBI Official Synonym Full Names: adenylate cyclase 5
• NCBI Official Symbol: Adcy5
• NCBI Protein Information: adenylate cyclase type 5
• UniProt Protein Name: Adenylate cyclase type 5
• Protein Family: Adenylate cyclase
• UniProt Gene Name: Adcy5
• UniProt Entry Name: ADCY5_RAT

NCBI Description

enzyme that catalyzes the formation of the secondary messenger cyclic adenosine monophosphate [RGD, Feb 2006]

Uniprot Description

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed:1409703). Mediates signaling downstream of ADRB1. Regulates the increase of free cytosolic Ca2+ in response to increased blood glucose levels and contributes to the regulation of Ca2+-dependent insulin secretion.