Rabbit anti-Human IgM (Immunoglobulin Mu Heavy Chain) Antibody | anti-IGHM antibody

IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker)

Cat. Num. AAA4381006

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: IgM (Immunoglobulin Mu Heavy Chain); IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker); AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region; anti-IGHM antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Clone Number: IGHM/3776R
• Specificity: Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 452

• Applicable Applications for anti-IGHM antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: 293T, Raji or hPBL cells. Human tonsil or spleen (IHC).
• Immunogen: Recombinant full-length human IGHM protein
• Cellular Localization: Cytoplasm, Cell Surface and Secreted
• Hu-chromosome Location: 14q32.33
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human spleen stained with IgM Recombinant Rabbit Monoclonal Antibody (IGHM/3776R).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human spleen stained with IgM Recombinant Rabbit Monoclonal Antibody (IGHM/3776R).)

SDS-Page

(SDS-PAGE Analysis Purified IgM Recombinant Rabbit Monoclonal Antibody (IGHM/3776R). Confirmation of Integrity and Purity of Antibody.)

References

1. Baryshnikov AIu. Gematol Transfuziol. 1990 Aug;35(8):4-7. 2. Martinova T.et al., In: Problems medical biotechnology and immunological infection diseases. Vol 11, 182-186, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 193806374
• UniProt Accession #: P01871; P20769
• Molecular Weight: 50-75kDa
• NCBI Official Full Name: Ig mu chain C region
• UniProt Protein Name: Ig mu chain C region
• UniProt Gene Name: IGHM
• UniProt Entry Name: IGHM_HUMAN