Western blot analysis of extracts of various cell lines, using TCF3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Rabbit TCF3 Antibody | anti-TCF3 antibody
TCF3 Rabbit pAb
Gene Names
TCF3; E2A; E47; ITF1; VDIR; TCF-3; bHLHb21
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
TCF3; TCF3 Rabbit pAb; E2A; E47; ITF1; VDIR; TCF-3; bHLHb21; anti-TCF3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Applicable Applications for anti-TCF3 antibody
Western Blot (WB)
Application Notes
WB 1:500 - 1:2000
Storage buffer
PBS with 0.02% sodium azide, pH7.3.
Immunogen
Recombinant protein of human TCF3
Positive Samples
HeLa, HCT116, Jurkat
Cellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western blot analysis of extracts of various cell lines, using TCF3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using TCF3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Related Product Information for anti-TCF3 antibody
This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW 67kDa/68kDa; Observed MW 70kDa
NCBI Official Full Name
Transcription factor E2-alpha
NCBI Official Synonym Full Names
transcription factor 3
NCBI Official Symbol
TCF3
NCBI Official Synonym Symbols
E2A; E47; ITF1; VDIR; TCF-3; bHLHb21
NCBI Protein Information
transcription factor E2-alpha; kappa-E2-binding factor; VDR interacting repressor; transcription factor ITF-1; helix-loop-helix protein HE47; transcription factor 3 variant 3; immunoglobulin transcription factor 1; vitamin D receptor-interacting repressor; class B basic helix-loop-helix protein 21; negative vitamin D response element-binding protein; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
UniProt Protein Name
Transcription factor E2-alpha
Protein Family
UniProt Gene Name
TCF3
UniProt Synonym Gene Names
BHLHB21; E2A; ITF1; bHLHb21; TCF-3
UniProt Entry Name
TFE2_HUMAN
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Product Notes
The TCF3 tcf3 (Catalog #AAA9143652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF3 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the TCF3 tcf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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