Rabbit SUMO3 Antibody | anti-SUMO3 antibody

SUMO3 Antibody

Cat. Num. AAA8504610

• Gene Names: SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: SUMO3; SUMO3 Antibody; SMT3A; Smt3B; SMT3H1; SUMO-3; anti-SUMO3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 141

• Applicable Applications for anti-SUMO3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:100-1:300
• Immunogen: A synthetic peptide of human SUMO3
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of MCF7 cellline, using SUMO3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embeddedHuman colon cancer tissue using SUMO3 antibody at dilution 1/100.)

Related Product Information for anti-SUMO3 antibody

Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1,-2 and-3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1,-2 or-3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1,-2 or-3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1,-2 or-3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IkappaB-alpha to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11).

Product Categories/Family for anti-SUMO3 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem. Sci. 28, 321-328. 2. Kim, K.I. et al. (2002) J. Cell Physiol. 191, 257-268. 3. Matunis, M.J. et al. (1996) J. Cell Biol. 135, 1457-1470. 4. Duprez, E. et al. (1999) J. Cell Sci. 112, 381-393. 5. Gostissa, M. et al. (1999) EMBO J. 18, 6462-6474. 6. Rodriguez, M.S. et al. (1999) EMBO J. 18, 6455-6461. 7. Desterro, J.M. et al. (1998) Mol. Cell 2, 233-239. 8. Tatham, M.H. et al. (2001) J. Biol. Chem. 276, 35368-35374. 9. Azuma, Y. et al. (2003) J. Cell Biol. 163, 477-487. 10. Li, Y. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 259-264. 11. Saitoh, H. and Hinchey, J. (2000) J. Biol. Chem. 275, 6252-6258.

NCBI and Uniprot Product Information

• NCBI GI #: 556695502
• NCBI GeneID: 6612
• NCBI Accession #: NP_001273345.1
• NCBI GenBank Nucleotide #: NM_001286416.1
• UniProt Accession #: P55854; Q53HI9; Q6FGD4; Q9BWR4; B2R5X4; B4DUW4
• Molecular Weight: 12kDa
• NCBI Official Full Name: small ubiquitin-related modifier 3 isoform 2
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 3
• NCBI Official Symbol: SUMO3
• NCBI Official Synonym Symbols: SMT3A; Smt3B; SMT3H1; SUMO-3
• NCBI Protein Information: small ubiquitin-related modifier 3
• UniProt Protein Name: Small ubiquitin-related modifier 3
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO3
• UniProt Synonym Gene Names: Smt3A

NCBI Description

This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]

Uniprot Description

Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).