Western Blot (WB)
(Western blot analysis of Hela cell lysateusing TGFA antibody.)
Rabbit Pro-TGF-alpha Antibody | anti-Pro-TGF-alpha antibody
Pro-TGF-alpha Antibody
Gene Names
TGFA; TFGA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
Pro-TGF-alpha; Pro-TGF-alpha Antibody; TFGA; TGF-alpha; anti-Pro-TGF-alpha antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
159
Applicable Applications for anti-Pro-TGF-alpha antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human TGFA
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Hela cell lysateusing TGFA antibody.)
Western Blot (WB)
(Western blot analysis of Hela cell lysateusing TGFA antibody.)
Related Product Information for anti-Pro-TGF-alpha antibody
Transforming growth factor alpha (TGF-alpha) is a member of the epidermal growth factor (EGF) family, sharing the same receptor, EGFR, and regulating cell proliferation, survival, and differention (1). Members of the family share an EGF-like domain of 45-60 amino acids characterized by the conservation of six regularly spaced cysteins, forming three disulfide bonds that function as their receptor binding domain. TGF-alpha was initially discovered in the media of retrovirally transformed fibroblasts, and it name comes from its ability to induce transformation in cultured fibroblasts (2). This transforming activity was later shown to require TGF-beta, which potentiates the activity of TGF-alpha through a separate receptor (3). Soluble TGF-alpha is released from its membrane-bound precusor, pro-TGF-alpha, following protolytic cleavage, but the membrane bound precursor is still able to bind and activate EGFR (4). Binding of soluble or membrane bound TGF-alpha to EGFR leads to receptor dimerization, tyrosine autophosphorylation, and activation of downstream signaling components. TGF-alpha and related peptides play an important role in the progression of cancer as well as in neuropathological processes (5, 6).
Product Categories/Family for anti-Pro-TGF-alpha antibody
References
1. Derynck, R. (1986) J. Cell Biochem. 32, 203-204. 2. de Larco, J.E. and Tordaro, G.J. (1978) Proc. Natl. Acad. Sci. USA 75, 4001-4005. 3. Roberts, A. B. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 5339-5343. 4. Wong, S. T. et al. (1989) Cell 56, 495-506. 5. Rusch, V. et al. (1996) Cytokine Growth Factor Rev. 7, 133-141. 6. Junier, M.P. (2000) Prog. Neurobiol. 62, 443-473.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17kDa
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
NCBI Description
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Research Articles on Pro-TGF-alpha
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Product Notes
The Pro-TGF-alpha tgfa (Catalog #AAA8501877) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pro-TGF-alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pro-TGF-alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the Pro-TGF-alpha tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pro-TGF-alpha, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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