Testing Data
(Western blot analysis of extracts of HeLacell lines, using POU2F1 antibody.)
Rabbit POU2F1 Antibody | anti-POU2F1 antibody
POU2F1 Antibody
Gene Names
POU2F1; OCT1; OTF1; oct-1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
POU2F1; POU2F1 Antibody; OCT1; OTF1; anti-POU2F1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
755
Applicable Applications for anti-POU2F1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human POU2F1
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Testing Data
(Western blot analysis of extracts of HeLacell lines, using POU2F1 antibody.)
Testing Data
(Western blot analysis of extracts of HeLacell lines, using POU2F1 antibody.)
Related Product Information for anti-POU2F1 antibody
Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6).
Product Categories/Family for anti-POU2F1 antibody
References
1. Sturm, R.A. et al. (1988) Genes Dev 2, 1582-99. 2. Mittal, V. et al. (1996) Mol Cell Biol 16, 1955-65. 3. Strubin, M. et al. (1995) Cell 80, 497-506. 4. Nakshatri, H. et al. (1995) J Biol Chem 270, 19613-23. 5. Zwilling, S. et al. (1994) Nucleic Acids Res 22, 1655-62. 6. Schonemann, M.D. et al. (1998) Adv Exp Med Biol 449, 39-53.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76kDa
NCBI Official Full Name
POU domain, class 2, transcription factor 1 isoform 2
NCBI Official Synonym Full Names
POU class 2 homeobox 1
NCBI Official Symbol
POU2F1
NCBI Official Synonym Symbols
OCT1; OTF1; oct-1B
NCBI Protein Information
POU domain, class 2, transcription factor 1
UniProt Protein Name
POU domain, class 2, transcription factor 1
Protein Family
UniProt Gene Name
POU2F1
UniProt Synonym Gene Names
OCT1; OTF1; Oct-1; OTF-1
NCBI Description
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]
Uniprot Description
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Research Articles on POU2F1
Similar Products
Product Notes
The POU2F1 pou2f1 (Catalog #AAA8501472) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU2F1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POU2F1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the POU2F1 pou2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU2F1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
