Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PDF protein (red) or empty vector (blue) stained using PDF antibody)
Mouse PDF Monoclonal Antibody | anti-PDF antibody
PDF antibody
FC: 1:100
WB: 1:500
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PDF protein (red) or empty vector (blue) stained using PDF antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PDF protein (red) or empty vector (blue) stained using PDF antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDF protein (Right) or empty vector (Left) detected with PDF antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDF protein (Right) or empty vector (Left) detected with PDF antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PDF protein using PDF antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PDF protein using PDF antibody)
NCBI and Uniprot Product Information
NCBI Description
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq, Nov 2008]
Uniprot Description
PDF: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Belongs to the polypeptide deformylase family.
Protein type: EC 3.5.1.88; Hydrolase; Mitochondrial
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: mitochondrion
Molecular Function: iron ion binding; peptide deformylase activity
Biological Process: translation; peptidyl-methionine modification; positive regulation of cell proliferation; N-terminal protein amino acid modification
Research Articles on PDF
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Product Notes
The PDF pdf (Catalog #AAA832023) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDF can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). IF: 1:100 FC: 1:100 WB: 1:500. Researchers should empirically determine the suitability of the PDF pdf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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