Rabbit PCNA Antibody | anti-PCNA antibody

PCNA Antibody

Cat. Num. AAA856728

• Gene Names: PCNA; ATLD2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Purification
• Synonyms: PCNA; PCNA Antibody; anti-PCNA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: Each vial at least contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.1% sodium azide.
• Sequence Length: 261

• Applicable Applications for anti-PCNA antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1000; IHC: 1:50-200
• Preparation and Storage: Store at 4 degree C or-20 degree C (with 50% glycerol); Minimize freeze-thaw cycles; Non-hazardous.

Testing Data

Testing Data #2

(Immunohistochemistry of paraffin-embedded Breast cancer using PCNA Antibody)

Related Product Information for anti-PCNA antibody

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Product Categories/Family for anti-PCNA antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 4505641
• NCBI GeneID: 5111
• NCBI Accession #: NP_002583.1
• NCBI GenBank Nucleotide #: NM_002592.2
• UniProt Accession #: P12004; B2R897; D3DW02
• Molecular Weight: 28,769 Da
• NCBI Official Full Name: proliferating cell nuclear antigen
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA
• UniProt Synonym Gene Names: PCNA

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.