Rabbit Tenascin-N Polyclonal Antibody | anti-TNN antibody

Tenascin-N Antibody

Cat. Num. AAA9611996

• Gene Names: TNN; TNW; TN-W
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Tenascin-N; Polyclonal Antibody; Tenascin-N Antibody; TENN_HUMAN; TN-N; TNN; anti-TNN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Tenascin-N Antibody detects endogenous levels of total Tenascin-N.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1299

• Applicable Applications for anti-TNN antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Tenascin-N, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from RPMI-8226, using Tenascin-N Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9611996 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9611996) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-TNN antibody

Function: Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1 (By similarity) (PubMed:17909022). Involved in neurite outgrowth and cell migration in hippocampal explants (By similarity). During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling (By similarity). In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327). Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells (PubMed:17909022).

Subcellular Location: Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity: Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested (PubMed:17909022, PubMed:19884327). In brain tumors, detected around the endothelial cell layer of the clood vessels (PubMed:19884327).

Subunit Structure: Homohexamer.

Similarity: Belongs to the tenascin family.

NCBI and Uniprot Product Information

• NCBI GI #: 62988324
• NCBI GeneID: 63923
• NCBI Accession #: NP_071376.1
• NCBI GenBank Nucleotide #: NP_071376.1
• UniProt Accession #: Q9UQP3
• Molecular Weight: Observed Molecular Weight: (Observed)144kD.; Predicted Molecular Weight: (Calculated)144kDa.
• NCBI Official Full Name: tenascin-N
• NCBI Official Synonym Full Names: tenascin N
• NCBI Official Symbol: TNN
• NCBI Official Synonym Symbols: TNW; TN-W
• NCBI Protein Information: tenascin-N
• UniProt Protein Name: Tenascin-N
• Protein Family: Tenascin
• UniProt Gene Name: TNN
• UniProt Synonym Gene Names: TNW1 PublicationManual assertion based on opinion iniRef.5; TN-W

Uniprot Description

Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1 () (PubMed:17909022). Involved in neurite outgrowth and cell migration in hippocampal explants (). During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling (). In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327). Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells (PubMed:17909022).