Western Blot (WB)
(Western blot analysis of extracts from HT29 cells, using Phospho-JUNB-S259 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Rabbit JUNB-S259 Antibody | anti-JUNB antibody
Phospho-JUNB-S259 Polyclonal Antibody
IHC: 1:50 - 1:100
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts from HT29 cells, using Phospho-JUNB-S259 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts from HT29 cells, using Phospho-JUNB-S259 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-JUNB-S259 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-JUNB-S259 antibody.)
NCBI and Uniprot Product Information
Uniprot Description
JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.
Protein type: Oncoprotein; Transcription factor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; chromatin
Molecular Function: protein binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription factor activity; transcription corepressor activity
Biological Process: response to drug; transcription initiation from RNA polymerase II promoter; response to light stimulus; response to peptide hormone stimulus; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; cellular response to hormone stimulus; trophectodermal cell differentiation; osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast proliferation; response to mechanical stimulus; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; vasculogenesis; positive regulation of cell differentiation; response to corticosterone stimulus
Research Articles on JUNB
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Product Notes
The JUNB junb (Catalog #AAA128384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-JUNB-S259 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JUNB-S259 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:100. Researchers should empirically determine the suitability of the JUNB junb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JUNB-S259, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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