Rabbit IL27 Antibody | anti-IL27 antibody

IL27 Antibody

Cat. Num. AAA9385530

• Gene Names: IL27; p28; IL30; IL-27; IL27A; IL-27A; IL27p28
• Reactivity: Human, Mouse, Rat
• Applications: Optimal dilutions/concentrations should be determined by the end user
• Purity: Immunogen affinity purified
• Synonyms: IL27; IL27 Antibody; EBI3; IL 27; IL 27 p28 subunit; IL 27A; IL 30; IL-27 subunit alpha; IL-27-A; IL27-A; IL27A; IL27A_HUMAN; IL27p28; IL30; Interleukin 27; Interleukin 27 subunit alpha; 28-KD subunit; Interleukin 30; Interleukin-27 subunit alpha; p28;; anti-IL27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Specificity: IL27 Antibody detects endogenous levels of total IL27
• Purity/Purification: Immunogen affinity purified
• Form/Format: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 243

• Applicable Applications for anti-IL27 antibody: Optimal dilutions/concentrations should be determined by the end user
• Application Notes: WB 1:500-1:2000; IHC 1:50-1:200.
• Function: Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.
• Immunogen: A synthesized peptide derived from human IL27
• Post-translational Modifications: O-glycosylated.
• Tissue Specificity: Expressed in monocytes and in placenta.
• Subunit Structure: Heterodimer with EBI3; not disulfide-linked. This heterodimer is known as interleukin IL-27
• Similarity: Belongs to the IL-6 superfamily.
• Sequence: 10 20 30 40 50 ; MGQTAGDLGW RLSLLLLPLL LVQAGVWGFP RPPGRPQLSL QELRREFTVS ; ; 60 70 80 90 100 ; LHLARKLLSE VRGQAHRFAE SHLPGVNLYL LPLGEQLPDV SLTFQAWRRL; ; 110 120 130 140 150 ; SDPERLCFIS TTLQPFHALL GGLGTQGRWT NMERMQLWAM RLDLRDLQRH ; ; 160 170 180 190 200 ; LRFQVLAAGF NLPEEEEEEE EEEEEERKGL LPGALGSALQ GPAQVSWPQL ; ; 210 220 230 240; LSTYRLLHSL ELVLSRAVRE LLLLSKAGHS VWPLGFPTLS PQP
• Preparation and Storage: Store at -20 °C.Stable for 12 months from date of receipt.

Testing Data TD

Related Product Information for anti-IL27 antibody

The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

NCBI and Uniprot Product Information

• NCBI GI #: 28416913
• NCBI GeneID: 246778
• NCBI Accession #: NP_663634.2
• NCBI GenBank Nucleotide #: NM_145659.3
• UniProt Accession #: Q8NEV9; Q6P676; A0N0L2
• Molecular Weight: 27 kDa
• NCBI Official Full Name: interleukin-27 subunit alpha
• NCBI Official Synonym Full Names: interleukin 27
• NCBI Official Symbol: IL27
• NCBI Official Synonym Symbols: p28; IL30; IL-27; IL27A; IL-27A; IL27p28
• NCBI Protein Information: interleukin-27 subunit alpha
• UniProt Protein Name: Interleukin-27 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL27
• UniProt Synonym Gene Names: IL27A; IL30; IL-27 subunit alpha; IL-27-A; IL27-A

NCBI Description

The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008]

Uniprot Description

IL27-alpha: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK- dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP- 10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation. Belongs to the IL-6 superfamily.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p12.1-p11.2

Cellular Component: extracellular region; extracellular space

Molecular Function: interleukin-27 receptor binding; receptor binding

Biological Process: positive regulation of interferon-gamma biosynthetic process; regulation of T cell proliferation; regulation of T-helper 1 cell differentiation