Western Blot (WB)
(Western blot analysis of Filamin A in lysates of 293, using Filamin A Antibody.)
Rabbit Filamin A Polyclonal Antibody | anti-FLN-A antibody
Filamin A Antibody
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of Filamin A in lysates of 293, using Filamin A Antibody.)
Western Blot (WB)
(Western blot analysis of Filamin A in lysates of 293, using Filamin A Antibody.)
Post Translational Modifications: Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA. Phosphorylation extent changes in response to cell activation. Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines (PubMed:24052262). Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome (PubMed:26766444).
Subunit Structure: Homodimer. Interacts with PDLIM2 (By similarity). Interacts with RFLNA and RFLNB (By similarity). Interacts with FCGR1A, FLNB, FURIN, HSPB7, INPPL1, KCND2, MYOT, MYOZ1, ARHGAP24, PSEN1, PSEN2 and ECSCR. Interacts also with various other binding partners in addition to filamentous actin. Interacts (via N-terminus) with MIS18BP1 (via N-terminus). Interacts (via N-terminus) with TAF1B. Interacts with TMEM67 (via C-terminus) and MKS1. Interacts (via actin-binding domain) with MICALL2 (via CH domain). Interacts (via filamin repeat 5) with SYK; docks SYK to the plasma membrane (PubMed:20713593). Interacts (via filamin repeats 19 and 21) with DRD3; increased PKA-mediated phosphorylation at Ser-2152. Interacts (via filamin repeat 21) with MAS1, AGTR1 and ADRA1D; increases PKA-mediated phosphorylation of FLNA at Ser-2152 (PubMed:26460884). Interacts (via filamin repeats 4, 9, 12, 17, 19, 21, and 23) with GP1BA (high affinity), ITGB7, ITGB2 and FBLIM1 (PubMed:19828450, PubMed:21524097, PubMed:25666618). Interacts with CEACAM1 (via cytoplasmic domain); inhibits cell migration and cell scattering by interfering with the interaction between FLNA and RALA (PubMed:16291724). Interacts with FOXC1 (PubMed:15684392).
Similarity: Comprised of a NH2-terminal actin-binding domain, 24 immunoglobulin-like internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation. Filamin repeat 20 interacts with filamin repeat 21 masking the ligand binding site on filamin repeat 21, resulting in an autoinhibited conformation (PubMed:17690686). The autoinhibition can be relieved by ligands like ITGB7 or FBLIM1 (PubMed:21524097). Filamin repeats 19 and 21 can simultaneously engage ligands (PubMed:21524097). Belongs to the filamin family.
NCBI and Uniprot Product Information
Predicted: 281 kDa
NCBI Description
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
Uniprot Description
FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking.
Protein type: Motility/polarity/chemotaxis; Actin-binding; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus
Molecular Function: Rho GTPase binding; protein homodimerization activity; Ral GTPase binding; Rac GTPase binding; transcription factor binding; small GTPase binding; actin filament binding; protein binding; signal transducer activity; protein kinase C binding; mu-type opioid receptor binding; glycoprotein binding; SMAD binding
Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; receptor clustering; mRNA transcription from RNA polymerase II promoter; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; positive regulation of transcription factor import into nucleus; cytoplasmic sequestering of protein; epithelial to mesenchymal transition; cilium biogenesis; blood coagulation; negative regulation of protein catabolic process
Disease: Fg Syndrome 2; Terminal Osseous Dysplasia; Cardiac Valvular Dysplasia, X-linked; Frontometaphyseal Dysplasia; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I
Research Articles on FLN-A
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Product Notes
The FLN-A flna (Catalog #AAA9611046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Filamin An Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Filamin A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the FLN-A flna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Filamin A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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