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Western Blot (WB) (Western blot analysis of extracts of Heartcell line, using IHH antibody.)

Rabbit Ihh Antibody | anti-Ihh antibody

Ihh Antibody

Gene Names
IHH; BDA1; HHG2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
Ihh; Ihh Antibody; IHH; BDA1; HHG2; Indian hedgehog protein C-product; HHG-2; anti-Ihh antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
411
Applicable Applications for anti-Ihh antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Fusion protein of human IHH
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of Heartcell line, using IHH antibody.)

Western Blot (WB) (Western blot analysis of extracts of Heartcell line, using IHH antibody.)
Related Product Information for anti-Ihh antibody
Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).
References
1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087. 2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114. 3. Zhang, X.M. et al. (2001) Cell 106, 781-792. 4. Adolphe, C. et al. (2004) Development 131, 5009-5019. 5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117. 6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45kDa
NCBI Official Full Name
indian hedgehog protein preproprotein
NCBI Official Synonym Full Names
indian hedgehog
NCBI Official Symbol
IHH
NCBI Official Synonym Symbols
BDA1; HHG2
NCBI Protein Information
indian hedgehog protein
UniProt Protein Name
Indian hedgehog protein
Protein Family
UniProt Gene Name
IHH
UniProt Synonym Gene Names
IHH

NCBI Description

This gene encodes a member of the hedgehog family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including an N-terminal fragment that is involved in signaling. Hedgehog family proteins are essential secreted signaling molecules that regulate a variety of developmental processes including growth, patterning and morphogenesis. The protein encoded by this gene specifically plays a role in bone growth and differentiation. Mutations in this gene are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Nov 2015]

Uniprot Description

Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) ().

Research Articles on Ihh

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Product Notes

The Ihh ihh (Catalog #AAA8505609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ihh Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ihh can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the Ihh ihh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ihh, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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