Rabbit FEN1 Antibody | anti-FEN1 antibody

FEN1 Antibody

Cat. Num. AAA8503985

• Gene Names: FEN1; MF1; RAD2; FEN-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: FEN1; FEN1 Antibody; FEN-1; MF1; RAD2; anti-FEN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 380

• Applicable Applications for anti-FEN1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human FEN1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-FEN1 antibody

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1, 2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4, 5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6, 7), post-translational modifications (8, 9), and subcellular re-localization in response to cell cycle or DNA damage (10).

Product Categories/Family for anti-FEN1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Shen, B. et al. (2005) Bioessays 27, 717-729. 2. Liu, Y. et al. (2004) Annu. Rev. Biochem. 73, 589-615. 3. Sakurai, S. et al. (2005) EMBO J. 24, 683-693. 4. Wu, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 1303-1308. 5. Gary, R. et al. (1999) J. Biol. Chem. 274, 4354-4363. 6. Brosh, R.M. et al. (2001) EMBO J. 20, 5791-5801. 7. Sharma, S. et al. (2004) J. Biol. Chem. 279, 9847-9856. 8. Henneke, G. et al. (2003) Oncogene 22, 4301-4313. 9. Hasan, S. et al. (2001) Mol. Cell 7, 1221-1231. 10. Qiu, J. et al. (2001) J. Biol. Chem. 276, 4901-4908.

NCBI and Uniprot Product Information

• NCBI GI #: 4758356
• NCBI GeneID: 2237
• NCBI Accession #: NP_004102.1
• NCBI GenBank Nucleotide #: NM_004111.5
• UniProt Accession #: P39748
• Molecular Weight: 43kDa
• NCBI Official Full Name: flap endonuclease 1
• NCBI Official Synonym Full Names: flap structure-specific endonuclease 1
• NCBI Official Symbol: FEN1
• NCBI Official Synonym Symbols: MF1; RAD2; FEN-1
• NCBI Protein Information: flap endonuclease 1
• UniProt Protein Name: Flap endonuclease 1
• UniProt Gene Name: FEN1
• UniProt Synonym Gene Names: MF1; hFEN-1

NCBI Description

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Uniprot Description

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.