Rabbit Bim Antibody | anti-Bim antibody

Bim Antibody

Cat. Num. AAA8504817

• Gene Names: BCL2L11; BAM; BIM; BOD
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: Bim; Bim Antibody; BAM; BIM; BOD; BimL; BimS; BimEL; BIM-beta6; BIM-beta7; BIM-alpha6; BCL2L11; anti-Bim antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 108

• Applicable Applications for anti-Bim antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human Bim
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of mouse eye tissue, using BIM antibody.)

Related Product Information for anti-Bim antibody

Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1, 2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1, 2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8, 9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.

Product Categories/Family for anti-Bim antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. O'Connor, L. et al. (1998) EMBO J 17, 384-95. 2. Hsu, S.Y. et al. (1998) Mol Endocrinol 12, 1432-40. 3. Bouillet, P. et al. (2002) Nature 415, 922-6. 4. Whitfield, J. et al. (2001) Neuron 29, 629-43. 5. Dijkers, P.F. et al. (2000) Curr Biol 10, 1201-4. 6. Ley, R. et al. (2003) J Biol Chem 278, 18811-6. 7. Puthalakath, H. et al. (1999) Mol Cell 3, 287-96. 8. Lei, K. and Davis, R.J. (2003) Proc Natl Acad Sci U S A 100, 2432-7. 9. Putcha, G.V. et al. (2003) Neuron 38, 899-914.

NCBI and Uniprot Product Information

• NCBI GI #: 323362959
• NCBI GeneID: 10018
• NCBI Accession #: NP_001191035.1
• NCBI GenBank Nucleotide #: NM_001204106.1
• UniProt Accession #: O43521; O43522; Q0MSE7; Q0MSE8; Q0MSE9; Q53R28; Q6JTU6; Q6T851; Q6TE14; Q6TE15; Q6TE16; A8K2W2
• Molecular Weight: 22kDa
• NCBI Official Full Name: bcl-2-like protein 11 isoform 11
• NCBI Official Synonym Full Names: BCL2 like 11
• NCBI Official Symbol: BCL2L11
• NCBI Official Synonym Symbols: BAM; BIM; BOD
• NCBI Protein Information: bcl-2-like protein 11
• UniProt Protein Name: Bcl-2-like protein 11
• UniProt Gene Name: BCL2L11
• UniProt Synonym Gene Names: BIM; Bcl2-L-11

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Uniprot Description

Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.